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2D electrophoresis Squall 01/08/04
    Hi, bit of a technical question.

    Does anybody know of a good kit to extract proteins from human fibroblasts to run on a 2d electrophoresis system?

    Thanks

      Clarification/Follow-up by rainbow1000 on 01/09/04 2:08 am:
      You could check out this site for BioRad:

      http://www.laboratorytalk.com/news/bie/bie116.html

      Clontech also provides kits for protein extraction.

      Clarification/Follow-up by Squall on 01/12/04 5:22 am:
      Thanks, hopefully the sample will run in the first dimention!

      Clarification/Follow-up by rainbow1000 on 01/12/04 8:53 am:
      I guess so. I have never personally used these kits, however, the AD mentions use of their kits for extracting proteins to be run on a 2-D electrophoresis system.

 
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